Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Biological Effects of Maslinic Acid on Human Epithelial Cells Used in Tissue Engineering

doi: 10.3389/fbioe.2022.876734

Figure Lengend Snippet: Generation of primary cultures of human skin keratinocytes using the explant technique (small tissue fragments were placed in direct contact with the culture surface to allow cells to migrate from the tissue to the culture flask). (A) : Phase contrast microscope images of primary keratinocytes migrating from the skin explants (labeled with asterisks) to the culture surface in each culture condition. Scale bars: 100 µm. (B) : Illustrative images of skin tissue explants cultured for 21 days in CTR and MA medium at a concentration of 5 μg/ml stained with hematoxylin-eosin. Some skin explants failing to generate any keratinocyte cultures are labeled with black arrows.

Article Snippet: A diaminobenzidine substrate kit (Vitro/Master Diagnostica) was used to detect antibody binding and samples were then counterstained for 15 s with Harry’s Hematoxylin (Panreac/Applichem, Barcelona, Spain).

Techniques: Microscopy, Labeling, Cell Culture, Concentration Assay, Staining